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Product

Regular Nucleic Acid Extraction Reagent

Sputum Nucleic Acid Extraction Reagent

Whole Blood Nucleic Acid Extraction Reagent

Bacterial Nucleic Acid Extraction Reagent

Veterinary Nucleic Acid Extraction Reagent

Ref No.

MTQ036

MTQP036

MTQB036

MTQM036

MTQ036A

Specification

36 Tests/Kit

Components

Quadruple Cuvette

36

36

36

36

36

Elution Cuvette

36

36

36

36

36

Magnetic Rod Sleeve

36

36

36

36

36

Sputum Liquefier

/

0.9mL/tube×2

/

/

/

Proteinase K

/

/

720μL/tube×1

/

720μL/tube×1

Specimen Requirements

Pharyngeal swabs, nasal swabs, oropharyngeal/oral secretions, and nasal/nasopharyngeal secretions, serum, plasma, and cervical exfoliated cells.

Sputum

Fresh or frozen whole blood

Pharyngeal swabs, nasal swabs, nasopharyngeal secretions, serum, plasma, and cervical exfoliated cells.

Animal samples such as whole blood, serum, plasma, saliva, nasal fluid, urine, oral and nasal swabs, environmental gauze, fecal swabs, animal tissues, feed pellets, etc.

Storage Conditions and Validity

The kit is transported and stored at 15-30℃ and valid for 12 months.

The kit can be transported at room temperature (15~30℃). The Sputum liquefier can be stable for 12 months stored at 2~30℃.

The kit can be transported at room temperature (15~30℃). The Proteinase K can be stable for 12 months stored at 2~30℃.

The kit is transported and stored at room temperature (15-30℃) and valid for 12months.

The kit is transported and stored at 10-30℃ and valid for 12 months.

Extraction Time

13-20 mins

Applicable Instruments

Singu20 nucleic acid extractor by Singuway.

Applicable Test Items

Available for all Singuway human test items.

Available for all Singuway veterinary test items.

Applications

RT-PCR, cDNA synthesis, Northern/ dot/ slot blot analysis, Microarrays, RNAseq

Clinical in vitro tests ofanimals

Product Name

Regular Nucleic Acid Extraction Reagent

SputumNucleic Acid Extraction Reagent

Whole Blood Nucleic Acid Extraction Reagent

BacterialNucleic Acid Extraction Reagent

Veterinary Nucleic AcidExtraction Reagent

Ref No.

MTQ036

MTQP036

MTQB036

MTQM036

MTQ036A

Extraction Sensitivity

The amplification sensitivity reached ≥100 copies/mL.

/

/

The amplification sensitivityof200 μL of novel coronavirus nucleic acid standard material was ≥100 copies/mL.

/

Average Yield

/

Extract 200μL sputumsample with≥1 μg of nucleic acid.

≥1μg of genomic DNAper100μL of blood

/

/

Nucleic Acid Purity

A260/A280ratio: 1.5~2.2.

OD260/OD280ratio: 1.7~2.1.

OD260/OD280ratio: 1.6~2.1.

A260/A280ratio: 1.5~2.2.

OD260/OD280 ratio:1.5 to 2.2.

Nucleic AcidExtraction Recovery Rate

90%~110%

≥90%

≥ 85%

90%~110%

90%~110%

Precision

The same sample was extracted 10 times with the same batch of reagents. Intra-batch Precision CV≤5%.

The coefficient of variation of in-batch concentration is ≤10%.

The coefficient of variation of in-batch concentration is ≤10%.

/

ct value, intra-batch CV value≤5%.

DNA Integrity

/

/

/

/

The extracted DNA bands were single, clear, with appropriate fragment size and no trailing bands or obvious degradation.