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Singuway Nucleic Acid Detection Kit
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Adenovirus

Description


  • Adenoviruses (AdVs) are common pathogens in human, causing diseases not only in airways, eyes, and intestine but also in the liver, urinary tract, and/or adenoids.
  • To date, more than 100 human AdV (HAdV) types have been isolated, characterized, and classified into seven species (A to G).
  • The two sole members of HAdV species F, HAdV-F40 and HAdV-F41, stand out as the only HAdVs with a pronounced gastrointestinal tropism. These so-called enteric AdVs are a leading cause of diarrhea and diarrhea-associated mortality in young children.
  • Diarrhea is estimated to cause ~530,000 deaths/year in children younger than 5 years worldwide.



Intended Use

The Detection Kit for Adenovirus (PCR-Fluorescence Probing) is provided for fast and qualitative detection of Adenovirus in samples extracted from swab specimens by means of Real-Time PCR for diagnosis of Adenovirus presence. 

*The test results are for clinical reference only and cannot be used as a basis for confirming or excluding cases alone.


Features


  • Highly specific detection for human adenoviruses (HAdV-A to HAdV-G) or gastrointestinal specie AdV-F 41
  • PCR buffer and polyase mix contain Uracil-DNA glycosylase (UDG) eliminating carryover contamination
  • Ready-to-use kit simplifies workflow & compatible with a wide range of real-time PCR devices
  • Rapid detection, the amplification can be completed within 30mins



Technical Specifications


REF No.

ADV001

Regulatory Status

CE IVD

Packing Specifications

48 Tests/ Kit, 96 Tests/Kit

Technology

Real-time PCR

Type Of Analysis

Qualitative

Sample Type

Nasopharyngeal Swab, Oropharyngeal Swabor Feces

Reaction Volume

30μL

Species

Human Adenoviruses (HAdV-A to HAdV-G)

Analytical Sensitivity

500 copies/mL

Precision

CV≤5%

Storage

-20±5℃in dark for 12 months

Main Components

PCR Buffer, Polyase Mix, Negative Control, Positive Control

Validated Extraction Method

Nucleic acid extraction kit (Singuway REF:MTQ064/MTQ096) is recommended.

Instruments

A real-time PCR instrument containing FAM and ROX fluorescence channels, such as Singuway Singu9600, Bio-Rad CFX96, ABI 7500, Thermo Fisher StepOnePlus.

Required Detection Channels

FAM, ROX

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