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Singuway Nucleic Acid Detection Kit
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Dengue Virus

Nucleic Acid Detection Kit for Dengue Virus (PCR-Fluorescence Probing)


Intended Use

This kit is used for the qualitative detection of dengue virus (including four serotypes I, II, III and IV) RNA in human serum and plasma samples in vitro. Dengue virus is a flavivirus in the Flaviviridae family. Dengue fever is an acute vector-borne disease caused by dengue virus (DV). Four serotypes of dengue virus (DENV-1, DENV-2, DENV-3, and DENV-4) have been isolated, all of which are pathogenic.

*The test results of this kit are for clinical reference only, and cannot be used alone to confirm or exclude cases, nor to screen normal populations. Experiment operators should have received professional training in gene amplification or molecular biology method detection, and have relevant experimental operation qualifications. Laboratories should have reasonable biosafety precautions and protection procedures.

 

Features

Highly specific detection for serotypes of dengue virus (DENV-1, DENV-2, DENV-3, and DENV-4)

PCR buffer and polyase mix contain Uracil-DNA glycosylase (UDG) and dNTPs eliminating carryover contamination

Ready-to-use kit simplifies workflow & compatible with a wide range of real-time PCR devices

Rapid detection, the amplification can be completed within 30mins

Technical Specifications

REF No.

DV001

Regulatory Status

CE IVD

Packing Specifications

48 tests/kit, 96 tests/kit

Technology

Real-time PCR

Type Of Analysis

Qualitative

Sample Type

Serum and Plasma

Reaction Volume

30μL

Species

Four serotypes I, II, III and IV

Analytical Sensitivity

1000 copies/mL

Precision

CV≤5%

Storage

-20±5℃ in darkfor 12 months

2~8℃ after opening the cover for2 weeks

Main Components

Name

48 tests/ kit

96 tests/ kit

Constituents

PCRBuffer

888μL ×1 Tube

888μL ×2 Tubes

Primers, probes, dNTPs

Polyase Mix

72μL×1 Tube

72μL×2 Tubes

DNA polymerase, Reverse Transcriptase, UDG

NegativeControl

800μL×1 Tube

800μL×2Tubes

Synthetic nucleic acid with target gene fragment

PositiveControl

800μL×1 Tube

800μL×2 Tubes

Synthetic nucleic acid with target gene fragment

Validated Extraction Method

General nucleic acid extraction kit (Singuway Ref No.: MTQ064/MTQ096) is recommended.

Instruments

A real-time PCR instrument containing at least two colors fluorescence channels, such as Singuway Biotech Inc. AccuRa-32, Bio-Rad CFX96, Thermo Fisher StepOnePlus, Tianlong Gentier 96E.

Required Detection Channels

FAM(DV),HEX (Internal Control)


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